Biolegend, 380402, Purified anti-human CD209 (DC-SIGN) Antibody, 100μg

CD209, known as Dendritic Cell-Specific Intercellular adhesion molecule 3 (ICAM-3)-Grabbing Nonintegrin (DC-SIGN), is a 44 kD type II transmembrane glycoprotein and a member of the C-type lectin family. CD209 is expressed on myeloid dendritic cells, placental macrophages, liver and placental endothelial cells. CD209 binds to ICAM-3 (CD50), ICAM-2 (CD102), and Butyrophilin (BTN2A1), and mediates dendritic cell migration and T cell proliferation. Importantly, CD209 is a receptor of HIV-1 and some other viruses (such as West Nile virus, hepatitis C virus, etc), and some bacteria or parasites. It plays a critical role in capturing and internalizing those pathogens. LSP1 (leukocyte-specific protein 1) interacts with the cytoplasmic domain of CD209 and mediates transport of HIV to the proteasome.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human CD209 protein, extracellular domain.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone A20120B can partially block the binding of clone 9E9A8 to target. It does not affect the binding of clone DCS-8C1, clone A20120F, and clone 14E3G7.
RRID: AB_2924571 (BioLegend Cat. No. 380402)
Structure: A 44 kD type II transmembrane glycoprotein and a member of the C-type lectin family
Distribution: Dendritic cells, Endothelial cells, Macrophages
Function: Functions in cell adhesion and pathogen recognition. This receptor recognizes a wide range of evolutionarily divergent pathogens with a large impact on public health, including leprosy and tuberculosis mycobacteria, the Ebola, hepatitis C, HIV-1 and Dengue viruses, and the SARS-CoV acute respiratory syndrome coronavirus.
Cell Type: Dendritic cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: Fauci A. 1996. Nature. 384:529-34. Granelli-Piperno A, et al. 2005. J Immunol. 175:4265-73. Lai WK, et al. 2006. Am J Pathol. 169:200-8.
Gene ID: 30835
UniProt: View information about CD209 on UniProt.org
Clone: A20120B
Regulatory Status: RUO
Other Names: Dendritic Cell-Specific Intercellular adhesion molecule 3 (ICAM-3)-Grabbing Nonintegrin (DC-SIGN), CLEC4L, DC-SIGN, DC-SIGN1, hDC-SIGN
Isotype: Rat IgG2b, κ