Biolegend, 382303, PE anti-human CTAG1B Antibody, 25tests

Cancer/testis antigen 1 also known as LAGE2 or LAGE2B is a protein that in humans is encoded by the CTAG1B gene. It is most often referenced by its alias New York esophageal squamous cell carcinoma 1(NY-ESO-1). Cancer/Testis Antigen 1B is a protein belonging to the family of Cancer Testis Antigens (CTA) that are expressed in a variety of malignant tumors at the mRNA and protein levels, but also restricted to testicular germ cells in normal adult tissues. The protein encoded by this gene is an antigen that is overexpressed in many cancers but it is also expressed in normal testis.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: hNY-ESO-1 protein (expressed by E. Coli)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2927832 (BioLegend Cat. No. 382303) AB_2927832 (BioLegend Cat. No. 382304)
Structure: CTAG1B is 180 amino acid long protein of 18 kD, containing epitopes of humoral and cellular responses in its glycine rich N terminal region and an extremely hydrophobic C terminal region.
Distribution: Normal expression is restricted to germ cells of the testis, ovary, and trophoblast cells of the placenta and upregulated in a variety of malignant tumors
Function: CTAG1B elicit both humoral and cellular immune responses in patients with cancer and therefore is a high priority target for cancer vaccine development and adoptive T-cell therapies.
Molecular Family: Protein Modifiers
Antigen References: Chen, Y.T. et al. 1997. Proc Natl Acad Sci USA 94:1914-8. De Smet, C. et al. 1999. Mol Cell Biol 19:7327-35. Gnjatic, S. et al. 2006. Adv Cancer Res 95:1-30. Caballero, O.L. et al. 2009. Cancer Sci 100:2014-21. Gjerstorff, M.F. et al. 2015. Oncotarget 6:15772-87. Esfandiary, A. et al. 2015. Immunotherapy 7:411-39.
Gene ID: 1485
UniProt: View information about CTAG1B on UniProt.org
Clone: W19067B
Regulatory Status: RUO
Other Names: CT6.1, CTAG, CTAG1, ESO1, LAGE2A, LAGE2B, NY-ESO-1
Isotype: Rat IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.