Biolegend, 382701, Purified anti-human CD22 Antibody, 25μg

CD22 is a 130 kD type I transmembrane glycoprotein also known as Siglec-2 and BL-CAM and is a member of the immunoglobulin superfamily (sialoadhesion subgroup). CD22 is expressed in the cytoplasm of pro-B and pre-B cells, and on the surface of mature B and activated B cells, but not on plasma cells. CD22 is present in the B cell receptor complex and associates with SHP-1, Syk, Lck, Lyn, and phospholipase Cγ1. A primary function of CD22 is thought to be in limiting antigen receptor signaling by modulating B cell activation threshold. CD22 has been shown to bind to CD45RO and CD75, although the natural ligands for this molecule remain controversial.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2927812 (BioLegend Cat. No. 382701) AB_2927812 (BioLegend Cat. No. 382702)
Structure: Ig superfamily, a type I glycosylated integral transmembrane protein, 120-130 kD
Distribution: B cells
Function: Adhesion, B-monocyte and B-T interactions
Ligand/Receptor: CD45RO, CD75
Biology Area: Cell Adhesion
Molecular Family: Adhesion Molecules
Antigen References: Clark E. 1993. J Immunol. 150:4715-8. Shan D, et al. 1995. J Immunol. 154:4466-75.
Gene ID: 933
UniProt: View information about CD22 on UniProt.org
Clone: A21052D
Regulatory Status: RUO
Other Names: BL-CAM, Siglec-2, Lyb8
Isotype: Mouse IgG2b, κ