Biolegend, 385006, PE anti-human CD27 Antibody, 100tests

CD27 is a 50-55 kD type I membrane protein also known as S152 and T14. It is a lymphocyte-specific member of the TNF-receptor superfamily. CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. CD27 binds to CD70 and plays an important role in costimulation of T cell activation, and regulation of B cell differentiation and proliferation. The cytoplasmic domains of CD27 have also been shown to interact with TRAF2 and TRAF5 to elicit NF-κB and SAPK/JNK activation.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Hu CD27 transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone S20020H does not block binding of clones M-T271, O323, or S20020G on target cells. Clone S20020H can block binding between CD27 and CD70 (CD27L).
RRID: AB_3083386 (BioLegend Cat. No. 385005) AB_3083386 (BioLegend Cat. No. 385006)
Structure: TNF-R superfamily, type I transmembrane glycoprotein, 50-55 kD
Distribution: Medullary thymocytes, T and B cell subsets, NK cells
Function: T cell costimulation
Interaction: TRAF2, TRAF5
Ligand/Receptor: CD70
Cell Type: B cells, Lymphocytes, NK cells, T cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: Camerini D, et al. 1991. J Immunol. 147:3156-60. Hintzen R, et al. 1994. Immunol Today. 15:307-11.
Gene ID: 939
UniProt: View information about CD27 on UniProt.org
Clone: S20020H
Regulatory Status: RUO
Other Names: S152, T14, TNFRSF7
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.