Biolegend, 385110, PE/Cyanine7 anti-human CD62L Antibody, 100tests

CD62L, also referred to as L-selectin or LECAM-1, is a 74-95 kD single chain type I glycoprotein. It is expressed on most peripheral blood B cells, subsets of T and NK cells, monocytes, granulocytes, and certain hematopoietic malignant cells. CD62L binds to carbohydrates present on certain glycoforms of CD34, glycam-1, and MAdCAM-1 and with a low affinity to anionic oligosaccharide sequences related to sialylated Lewis X (sLex, CD15s) through its C-type lectin domain. CD62L is important for the homing of naïve lymphocytes to high endothelial venules in peripheral lymph nodes and Peyer's patches. It also plays a role in leukocyte rolling on activated endothelial cells.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CD62L transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone S21001C does not block the binding of clone DREG-56 on target cells.Clone S21001C may be more sensitive in detecting CD62L surface expression level changes.
RRID: AB_3683426 (BioLegend Cat. No. 385109)
Structure: Selectin, single chain glycoprotein, 74-95 kD
Distribution: Majority of B cells, naïve T cells, subset of memory T and NK cells, monocytes, granulocytes, thymocytes
Function: Leukocyte homing, leukocyte tethering, rolling
Ligand/Receptor: CD34, GlyCAM, MAdCAM-1
Cell Type: B cells, Granulocytes, Lymphocytes, Monocytes, Neutrophils, NK cells, T cells
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Kishimoto T, et al. 1990. P Natl Acad Sci USA. 87:2244. Kishimoto T, et al. 1991. Blood. 78:805-11.
Gene ID: 6402
UniProt: View information about CD62L on UniProt.org
Clone: S21001C
Regulatory Status: RUO
Other Names: L-selectin, LECAM-1, LAM-1, Leu-8, TQ-1
Isotype: Mouse IgG1, κ