Biolegend, 385229, Brilliant Violet 785™ anti-human CD25 Antibody, 25tests

CD25 is a 55 kD transmembrane protein also known as interleukin-2 receptor alpha chain. CD25 in combination with CD122 and CD132 form a high affinity IL-2 receptor that binds to IL-2, playing a crucial role in activation of JAK1/JAK3 and STAT5 signaling. It is expressed on activated T cells as well as on CD4+ T regulatory cells, some progenitor B-cells and activated monocytes.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CD25 transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool).
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 785™ excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Clone S20019D can block binding of clone M-A251 on target cells and is blocked by M-A251.Clone S20019D does not block binding of clones S20019C, BC96, and TÜ69 on target cells.Clone S20019D does not affect binding of IL-2 to the receptor. Clone S20019D shows comparable CD25 staining on 4% formaldehyde-fixed cells compared to unfixed.
RRID: AB_3720549 (BioLegend Cat. No. 385229) AB_3720549 (BioLegend Cat. No. 385230)
Structure: 55 kD transmembrane protein
Distribution: Activated T cells, T regulatory cells, monocytes
Function: IL-2 receptor
Interaction: CD122, CD132
Ligand/Receptor: IL-2
Cell Type: Lymphocytes, T cells, Tregs
Biology Area: Cell Biology, Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Waldmann T. 1991. J Biol Chem. 266:2681-4. Sakaguchi S, et al. 1995. The Journal of Immunology. 155:1151-64.
Gene ID: 3559
UniProt: View information about CD25 on UniProt.org
Clone: S20019D
Regulatory Status: RUO
Other Names: interleukin-2 receptor alpha chain, interluekin-2 receptor, IL2RA, IL-2Rα
Isotype: Mouse IgG1, κ