Biolegend, 389604, PE anti-human CD158k (KIR3DL2) Antibody, 100tests

CD158 molecules, also known as KIRs (killer cell immunoglobulin-like receptors), are a family of transmembrane proteins with either two (KIR2D) or three (KIR3D) Ig-like extracellular domains. Some KIRs with long cytoplasmic domains contain ITIMs and possess inhibitory functions and others with short cytoplasmic region lack ITIM and have activation functions. 15 polymorphic KIR genes have been reported in humans. CD158k is a 70 kD three extracellular Ig domain glycoprotein that presents as a disulphide-bonded homodimer extracellularly. The intracellular long cytoplasmic tail of CD158k contains two ITIM domains. It is expressed primarily on a subset of NK cells and a subset of T cells. KIR3DL2 binds to a number of different MHC class 1 molecules depending on the viral peptide sequence presented.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: KIR3DL2-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Based on in-house testing on different KIR-expressing transfectants, clone S22025B does not interact with other KIR protein family members.
RRID: AB_3699043 (BioLegend Cat. No. 389603) AB_3699043 (BioLegend Cat. No. 389604)
Structure: 70 kD three Ig-like extracellular domain glycoprotein
Distribution: Subset of NK cells, subset of T cells
Function: NK Cell regulation
Ligand/Receptor: MHC Class 1 molecules
Cell Type: Lymphocytes, NK cells, T cells
Biology Area: Cell Biology, Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: Colonna M. et al. 1995. Science. 268:405-8. Lanier LL. 2005. Annu Rev Immunol. 23:225-74. Hansasuta P, et al. 2004. Eur J immunol. 34:1673-9.
Gene ID: 3812
UniProt: View information about CD158k on UniProt.org
Clone: S22025B
Regulatory Status: RUO
Other Names: NKAT4, Killer Cell Immunoglobulin-Like Receptor 3DL2
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.