Biolegend, 390553, PE anti-human CD202b (Tie2/Tek) Antibody, 25tests

CD202b, also known as Tie2 or TEK, is a 145 kD type I transmembrane protein. It is a member of the receptor tyrosine kinase (RTK) family of proteins and is expressed by endothelial cells and their progenitors, quiescent hematopoietic stem cells (HSCs), and a subpopulation of monocytes. Angiopoietin-1 (Ang-1) is an activator of CD202b to promote, maintain, and stabilize mature vessels and to maintain HSCs in quiescent state. Ang-2 is another ligand of CD202b, which is involved in postnatal angiogenesis and in antagonizing the effects of Ang-1. Tie2 binds to Ang-4 as well.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant extracellular domain of human Tie2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone A19069E partially block the staining of reference clone 33.1 ​Recombinant protein completely blocks the staining of test clone A19069E
RRID: AB_3675121 (BioLegend Cat. No. 390553) AB_3675121 (BioLegend Cat. No. 390554)
Structure: 145 kD type I transmembrane protein, receptor tyrosine kinase (RTK) family
Distribution: Endothelial cells, subsets of hematopoietic stem cells and and a subset of monocytes
Function: Angiogenesis, hemotopoiesis, monocytes chemotaxis
Interaction: Endothelial cells, hematopoietic stem and progenitors, monocytes
Ligand/Receptor: Angiopoietin-1 (Ang-1), Ang-2, and Ang-4
Cell Type: Endothelial cells, Hematopoietic stem and progenitors, Monocytes
Biology Area: Angiogenesis, Cell Biology, Immunology
Molecular Family: CD Molecules
Antigen References: De Palma M, et al. 2005. Cancer Cell. 8:211-26. Shaw JP, et al. 2004. Blood Cells Mol Dis. 32:168-75. Hsu HC, et al. 2000. Blood. 96:3757-62. Arai F, et al. 2004. Cell. 118:149-61.
Gene ID: 7010
UniProt: View information about CD202b on UniProt.org
Clone: A19069E
Regulatory Status: RUO
Isotype: Mouse IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.