Biolegend, 393713, Brilliant Violet 421™ anti-human CD110 Antibody, 25tests

CD110 is a type I transmembrane glycoprotein which functions as a receptor for thrombopoietin; binding causes the proliferation and differentiation of megakaroyocytes in addition to the production of platelets and protection of stem cells from apoptosis. CD110 is primarily expressed on hematopoietic stem cells, hematopoietic precursor cells, cells of the megakaryocytic lineage, and platelets.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CD110 transfectants
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
RRID: AB_2750508 (BioLegend Cat. No. 393713) AB_2750509 (BioLegend Cat. No. 393714)
Structure: Type I transmembrane glycoprotein
Distribution: Hematopoietic stem cells, a subfraction of hematopoietic precursor cells, cells of the megakaryocytic lineage, and platelets.
Function: Megakaryocytopoiesis and platelet formation
Interaction: THPO, ATXN2L, JAK2
Ligand/Receptor: Thrombopoietin
Cell Type: Hematopoietic stem and progenitors, Megakaryocytes, Platelets
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: Ninos JM, et al. 2006. J. Transl. Med. 16:4-9. Broudy VC, et al. 1996. Blood. 88:2026-32. Wang X, et al. 2016. Blood. 127:3398-409.
Gene ID: 4352
UniProt: View information about CD110 on UniProt.org
Clone: S16017E
Regulatory Status: RUO
Other Names: TPOR, c-MPL, MPLV
Isotype: Mouse IgG2a, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.