Biolegend, 394102, Purified anti-human CD133 Antibody, 100μg

CD133, also known as Prominin-1 and AC133 antigen, is a 120 kD pentaspan glycoprotein with 5 transmembrane domains. CD133 was initially described as a surface antigen specific for human hematopoietic stem cells and as a marker for murine neuroepithelial cells and some embryonic epithelia. Later on, CD133 was found on other stem cells, including endothelial progenitor cells, glioblastomas, neuronal, and glial stem cells. In addition to stem cells for normal tissue, CD133 was found on cancer cells, such as some leukemia cells and brain tumor cells. Although the biological function of CD133 is not completely understood, CD133 has been extensively used as a stem cell marker for normal and cancerous tissues.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CD133 transfectants
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: In-house testing suggests that clone S16016E blocks clone 293C3 but not clone 7 that are also raised against human CD133.
RRID: AB_2750456 (BioLegend Cat. No. 394101) AB_2750457 (BioLegend Cat. No. 394102)
Structure: 120 kD pentaspan transmembrance glycoprotein with 5 transmembrane domains
Distribution: Hematopoietic stem cells and progenitor cells, fetal liver cells, tissue specific stem cells or progenitor cells such as renal and prostate, a variety of tumor cells
Function: May play a role in cell differentiation, proliferation, and apoptosis
Interaction: NAT8; NAT8B; CDHR1
Cell Type: Hematopoietic stem and progenitors
Biology Area: Cancer Biomarkers, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: Yin AH, et al. 1997. Blood. 90:5002. Miraglia S, et al. 1997. Blood. 90:5013. Bühring HJ, et al. 1999. Ann. NY Acad. Sci. 872:25.
Gene ID: 8842
UniProt: View information about CD133 on UniProt.org
Clone: S16016E
Regulatory Status: RUO
Other Names: Prominin-1, PROM1, AC133
Isotype: Mouse IgG2a, κ