Biolegend, 394208, Alexa Fluor® 647 anti-human IL12RB2 Antibody, 100tests

IL12RB2 is a type I transmembrane protein that foms an heterodimer with IL12RB1 to produce the high affinity IL-12 receptor complex. IL12RB2 is expressed by activated T cells, TH1 cells, NK cells, B cells and plasma cells. Variants of this gene are associated with predisposition to various immune-related diseases.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human IL12RB2 transfected cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Product Citations: Preechanukul A, et al. 2023. iScience. 107234:26. PubMed
RRID: AB_2894595 (BioLegend Cat. No. 394207) AB_2894595 (BioLegend Cat. No. 394208)
Structure: Type I transmembrane protein, 3 isoforms, foms an heterodimer with IL12RB1
Distribution: Activated T cells, TH1 cells, NK cells, B cells, plasma cells
Function: Role in TH1 differentiation
Ligand/Receptor: IL-12
Cell Type: B cells, NK cells, Plasma cells, T cells, Th1
Biology Area: Immunology
Molecular Family: Cytokine/Chemokine Receptors
Antigen References: LaMere SA, et al. 2017. J Immunol. 199:3158 Prigione I, et al. 2016. Immunobiology. 221:291 de Paus RA, et al. 2013. Mol Immunol. 56:380 Airoldi I, et al. 2008. Blood. 112:750
Gene ID: 3595
UniProt: View information about IL12RB2 on UniProt.org
Clone: S16020B
Regulatory Status: RUO
Other Names: Interleukin-12 receptor subunit beta-2
Isotype: Mouse IgG2a, κ