Biolegend, 395727, Brilliant Violet 421™ anti-human β2-microglobulin Antibody, 25tests

β2-microglobulin (β2M) is a 12 kD nonpolymorphic Ig like protein. It is a non-membrane-anchored glycoprotein and is noncovalently associated with 39-44 kD polymorphic heavy chains of MHC class I molecules to form HLA class I antigen complex. In association with HLA class I, β2M is expressed on all leukocytes, platelets, endothelial cells, and epithelial cells. β2M plays an essential role both in governing MHC class I molecules stability and in promoting antigen binding and presenting the antigen to CD3/TCR complex of CD8 + T cells.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human Beta2-microglobulin
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Clone A17082A partially cross-blocks anti-human ß2-microglobulin clone 2M2 and does not block anti-human HLA-A,B,C clone W6/32 staining, based on in-house testing.
RRID: AB_3083395 (BioLegend Cat. No. 395727) AB_3083395 (BioLegend Cat. No. 395728)
Structure: 12 KD, Ig like structure
Distribution: Leukocytes, platelets, endothelial cells, epithelial cells and other nucleated cells.
Function: Form MHC class I
Interaction: MHC class I heavy chain and other MHC I like molecules.
Cell Type: Antigen-presenting cells, Endothelial cells, Epithelial cells, Leukocytes, Platelets
Biology Area: Cancer Biomarkers, Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: Engelhard VH. 1994. Curr. Opin. Immunol. 6:13. Williams DB, et al. 1989. J. Immunol. 142:2796. Danliczyk UG and TL. Delovitch. 1994. J. Immunol. 153:3533. Williams A, et al. 2002. Tissue Antigens. 59:3.
Gene ID: 567
UniProt: View information about beta2-microglobulin on UniProt.org
Clone: A17082A
Regulatory Status: RUO
Other Names: β2M, β2-M, beta2-microglobulin b2-M, b2M
Isotype: Mouse IgG1, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.