Biolegend, 395810, PerCP/Cyanine5.5 anti-human β2-microglobulin Antibody, 100tests

β2-microglobulin (β2M) is a 12 kD nonpolymorphic Ig like protein. It is a non-membrane-anchored glycoprotein and is noncovalently associated with 39-44 kD polymorphic heavy chains of MHC class I molecules to form HLA class I antigen complex. In association with HLA class I, β2M is expressed on all leukocytes, platelets, endothelial cells, and epithelial cells. β2M plays an essential role both in governing MHC class I molecules stability and in promoting antigen binding and presenting the antigen to CD3/TCR complex of CD8 + T cells.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human Beta2-microglobulin
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Based on in-house testing, staining for clone A17082E is not blocked by clone W6/32 (anti-HLA-A,B,C) and is only partially blocked by clone 2M2 also raised against human ß2-microglobulin.
RRID: AB_2810595 (BioLegend Cat. No. 395809) AB_2810596 (BioLegend Cat. No. 395810)
Structure: 12KD, Ig like structure
Distribution: Leukocytes, platelets, endothelial cells, epithelial cells and other nucleated cells.
Function: Form MHC class I
Interaction: MHC class I heavy chain and other MHC I like molecules.
Cell Type: Endothelial cells, Epithelial cells, Leukocytes, Platelets
Antigen References: Engelhard VH. 1994. Curr Opin Immunol. 6:13. Williams DB, et al. 1989. J Immunol. 142:2796. Danliczyk UG and TL. Delovitch. 1994. J Immunol. 153:3533. Williams A, et al. 2002. Tissue Antigens. 59:3.
Gene ID: 567
UniProt: View information about beta2-microglobulin on UniProt.org
Clone: A17082E
Regulatory Status: RUO
Other Names: β2M, β2-M, beta2-microglobulin b2-M, b2M
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.