Biolegend, 396406, PE anti-human/mouse Granzyme B Recombinant Antibody, 100tests

Granzyme B is a 32 kD serine protease, also known as granzyme-2, serine protease B, CCP1, Asp-ase, and CTLA-1. Granzyme B is abundantly stored in the granules of cytotoxic T lymphocytes and NK cells. Low level of expression has been reported in granulocytes, B cells, and activated dendritic cells. Granzyme B is crucial for rapid induction of cell death and apoptosis through interaction with mannose-6-phosphate receptor.
100tests
Verified Reactivity: Human, Mouse
Antibody Type: Recombinant
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone QA18A28 is compatible with Fixation buffer (Cat. No. 420801) and Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002) in both C57BL/6 splenocytes and human PBMC. QA18A28 is also compatible with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401).
Product Citations: Gao X, et al. 2023. Front Immunol. 14:1142428. PubMed Ajith A, et al. 2021. Front Immunol. 12:687715. PubMed Wei M, et al. 2022. Front Immunol. 13:1017574. PubMed Chen X, et al. 2022. J Extracell Vesicles. 11:e12279. PubMed Zuo S, et al. 2021. J Immunother Cancer. 9:. PubMed Lee W, et al. 2021. J Virol. 95:e0053021. PubMed
RRID: AB_2801074 (BioLegend Cat. No. 396405) AB_2801075 (BioLegend Cat. No. 396406)
Structure: 32 kD serine protease
Distribution: Cytotoxic T cells, NK cells, and neutrophils, low on granulocytes, B cells and activated dendritic cells
Function: Granzyme B is able to induce target cell apoptosis by activating caspase independent pathways. Granzyme B is induced in CD8+ T lymphocytes with ConA/ IL-2 and CD4+ T lymphocytes with anti CD3/CD28 or CD3/CD46.
Interaction: Caspase-3
Ligand/Receptor: Mannose-6-phosphate receptor
Cell Type: Neutrophils, T cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family: Enzymes and Regulators, Proteases
Antigen References: Estebanez-Perpina E, et al. 2000. Biol Chem. 381:1203. Griffiths GM. And S. Isaaz, et al. 1993. J. Cell Biol. 120:885. Spaeny-Dekking EH, et al. 1998. J. Immunol. 160:3610. Wagner C, et al. 2008. Mol. Immunol. 45:1761.
Gene ID: 300214939
UniProt: View information about Granzyme B on UniProt.org
Clone: QA18A28
Regulatory Status: RUO
Other Names: Granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-aseGranzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-ase
Isotype: Rat IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.