Biolegend, 398326, Brilliant Violet 421™ anti-human CD32B/C Antibody, 100tests

CD32B (FCGR2B) and CD32C (FCGR2C) are 40 kDa, type I transmembrane proteins that are members of the Ig superfamily of low-affinity immunoglobulin gamma Fc receptors. CD32B has a cytoplasmic tail that contains an immunoreceptor tyrosine-based inhibition motif (ITIM), while CD32C contains an immunoreceptor tyrosine-based activation motif (ITAM). CD32B and CD32C are low affinity receptor for monomeric IgG but also bind IgG complexes. CD32B and CD32C are expressed on B cells, subsets of monocytes,macrophages and granulocytes, platelets, mast cells, and is a negative regulator of cell activation, proliferation, endocytosis, phagocytosis, and degranulation.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Human Fc gamma RIIB/C (CD32b/c) Protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: As the extracellular region of CD32B and CD32C are identical, this Ab recognizes both isoforms. Does not crossreact with CD32A.
RRID: AB_3106181 (BioLegend Cat. No. 398325) AB_3106181 (BioLegend Cat. No. 398326)
Structure: Type I transmembrane protein, 40 kDa, member of the Ig superfamily
Distribution: B cells, subsets of monocytes,macrophages and granulocytes, platelets, mast cells.
Function: Negatively regulates cell activation, proliferation, endocytosis, phagocytosis, and degranulation
Ligand/Receptor: Fc region of complex or aggregated immunoglobulins gamma
Cell Type: B cells, Dendritic cells, Granulocytes, Monocytes, Platelets
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Fc Receptors
Antigen References: Bruhns P, et al. 2009. Blood. 113: 3716. Bewarder N, et al. 1996. Mol Cell Biol. 16: 4735. Descours B, et al. 2017. Nature. 543: 564. Tomiyama Y, et al. 1992. Blood. 80: 2261. Indik Z, et al. 1991. J Clin Invest. 88: 1766. Ramsland PA, et al. 2011. J Immunol. 187: 3208. Hogarth PM and Pietersz GA. 2012. Nat Rev Drug Discov. 11: 311. Bournazos S, et al. 2009. J Immunol. 182: 8026. Maxwell KF, et al. 1999. Nat Struct Biol. 6: 437. Sandilands GP, et al. 1997. Immunology. 91: 204. Ghazizadeh S, et al. 1994. J Biol Chem. 269: 8878. Gillis C, et al. 2014. Front Immunol. 5: 254.
Gene ID: 22139103
UniProt: View information about FCGR2B+OR+FCGR2B on UniProt.org
Clone: S18005H
Regulatory Status: RUO
Other Names: FCGR2B, FCgRIIB, FCGR2C, FCRIIC, IGFR2, CD32B, CD32C, FCG2, FCGR2, IGFR2, FcRII-c, CDW32
Isotype: Mouse IgG1, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.