Biolegend, 399505, FITC anti-human CD64 Antibody, 25tests

CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcR I. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human CD64-Fc (1257-FC-050)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: In-house testing suggests that clones S18012C and 10.1 do not cross-block each other and recognize different epitopes. Clone 10.1 recognizes the EC3 epitope of CD64. While both CD64 subunits contain EC3 domains, clone 10.1 preferentially binds to CD64A (FcγRIA) but not CD64B (FcγRIB).
Product Citations: Solier S, et al. 2023. Nature. 617:386. PubMed Yun H, et al. 2024. Leukemia. 1688:38. PubMed Li F, et al. 2022. Front Pharmacol. 13:1001552. PubMed
RRID: AB_2861007 (BioLegend Cat. No. 399505) AB_2861008 (BioLegend Cat. No. 399506)
Structure: Ig superfamily, type I glycoprotein, 72 kD
Distribution: Monocytes, macrophages, dendritic cells, activated granulocytes
Function: Phagocytosis, ADCC
Ligand/Receptor: IgG receptor
Cell Type: Dendritic cells, Granulocytes, Macrophages, Monocytes
Biology Area: Immunology, Inhibitory Molecules
Molecular Family: CD Molecules, Fc Receptors
Antigen References: HHulett MD & Hogarth PM. 1994. Adv Immunol. 57:1-127. van de Winkel JG & Capel PJ. 1993. Immunol Today. 14:215-21.
Gene ID: 2209
UniProt: View information about CD64 on UniProt.org
Clone: S18012C
Regulatory Status: RUO
Other Names: FcγRI, FcR I
Isotype: Mouse IgG1, κ