Biolegend, 400103, Biotin Mouse IgG1, κ Isotype Ctrl Antibody, 50μg

The MOPC-21 immunoglobulin has unknown specificity. The isotype of this antibody is mouse IgG1, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
50μg
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The immunoglobulin was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest.
Application Notes: Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA)
Application References(PubMed link indicates BioLegend citation): Carlsten M, et al. 2007. Cancer Res. 67:1317. PubMed Smed-Sörensen A, et al. 2008. Blood 111:5037. PubMed (FA) Bunesmann MM, et al. 2011. Am. J. Respir. Cell. Mol. Biol. Epub. PubMed Matsuyama T, et al. 2005. Infect. Immun. 73:1044. (IF) Correia DV, et al. 2011. Blood 118:992. (FC) PubMed Lian IA, et al. 2011. Placenta. 32:823. PubMed Bufe B, et al. 20015. J Biol Chem. 290:7369. PubMed
Product Citations: Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed Suthar J, et al. 2023. Nanoscale Horiz. 8:460. PubMed Chen H, et al. 2022. Cell Prolif. 55:e13255. PubMed Martínez P, et al. 2022. Nat Neurosci. 25:1597. PubMed Suthar J, et al. 2020. Anal Chem. 92:4082. PubMed Su K, et al. 2016. J Immunol. 197: 4163 - 4176. PubMed Wang Y, et al. 2016. Cell Death Dis. 7:e2458. PubMed Suthar J, et al. 2022. Anal Chem. 94:2465. PubMed Halkias J, et al. 2019. J Clin Invest. 130:3562. PubMed Georg P, et al. 2022. Cell. 185:493. PubMed Freeman DW, et al. 2018. Diabetes. 67:2377. PubMed Kämpe A, et al. 2021. PLoS One. 16:e0257442. PubMed
RRID: AB_326427 (BioLegend Cat. No. 400103) AB_326427 (BioLegend Cat. No. 400104)
Gene ID: NA
Clone: MOPC-21
Regulatory Status: RUO
Isotype: Mouse IgG1, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.