Biolegend, 400119, APC Mouse IgG1, κ Isotype Ctrl Antibody, 25μg

The MOPC-21 immunoglobulin has unknown specificity. The isotype of this antibody is mouse IgG1, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
25μg
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The immunoglobulin was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA)
Application References(PubMed link indicates BioLegend citation): Carlsten M, et al. 2007. Cancer Res. 67:1317. PubMed Smed-Sörensen A, et al. 2008. Blood 111:5037. PubMed (FA) Bunesmann MM, et al. 2011. Am. J. Respir. Cell. Mol. Biol. Epub. PubMed Matsuyama T, et al. 2005. Infect. Immun. 73:1044. (IF) Correia DV, et al. 2011. Blood 118:992. (FC) PubMed Lian IA, et al. 2011. Placenta. 32:823. PubMed Bufe B, et al. 20015. J Biol Chem. 290:7369. PubMed
Product Citations: Garcia–Perez JE, et al. 2019. Front Immunol. 10:998. PubMed Redka DS, et al. 2018. Mol Biol Cell. 29:53. PubMed Liisborg C, et al. 2022. Acta Ophthalmol. :3. PubMed Herrero C, et al. 2023. Biomedicines. 11:. PubMed Feng Y, et al. 2023. Acta Pharm Sin B. 13:598. PubMed Hurtado P, et al. 2023. Front Cell Dev Biol. 11:1076432. PubMed Hu X, et al. 2023. Nat Commun. 14:2020. PubMed Hu X, et al. 2023. Nat Biotechnol. . PubMed Yen FC, et al. 2023. Nat Commun. 14:2942. PubMed Shao L, et al. 2021. Cell Death Discov. 7:145. PubMed Bhatt RS, et al. 2021. Cancer Immunol Res. 9:156. PubMed Kemble S, et al. 2022. Blood Adv. 6:2932. PubMed Connor T, et al. 2022. Methods Mol Biol. 2475:47. PubMed Market M, et al. 2022. Int J Mol Sci. 23: . PubMed Cao B, et al. 2022. Nat Commun. 13:6203. PubMed McCartin C, et al. 2022. Cancers (Basel). 14:. PubMed Lokmic Z, et al. 2015. J Vis Exp. 99: 52691. PubMed Miao L, et al. 2020. Theranostics. 0.7625. PubMed Soriani A, et al. 2017. Oncoimmunology. 6:e1264564. PubMed de Tymowski C et al. 2019. Cell reports. 27(3):762-775 . PubMed Grayson KA, et al. 2021. Mol Cancer Ther. 20:833. PubMed Harkins S, et al. 2014. J Virol. 55:5807. PubMed Kubo M, et al. 2018. Oncol Rep. 39:417. PubMed Horii M, et al. 2021. Sci Rep. 11:5877. PubMed Botha J, et al. 2021. Biomedicines. 9:. PubMed Hemkemeyer SA, et al. 2020. J Biol Chem. 296:100136. PubMed Snyder J, et al. 2014. PLoS One. 9:107257. PubMed Perdiguero P, et al. 2019. Cell Rep. 29:4223. PubMed Zhang D, et al. 2020. Oncoimmunology. 9:1744921. PubMed Liu S, et al. 2021. Mol Ther Oncolytics. 21:303. PubMed Perelman SS, et al. 2021. Nat Microbiol. 6:731. PubMed Masuda T, et al. 2022. iScience. 25:104781. PubMed Cui L, et al. 2021. Am J Reprod Immunol. 86:e13436. PubMed Ma J, et al. 2021. Curr Protoc. 1:e144. PubMed Market M, et al. 2020. Front Immunol. 1.127083333. PubMed Maluski M, et al. 2019. J Clin Invest. 129:5108. PubMed Colliou N, et al. 2018. Gut Microbes. 9:279. PubMed Sun W, et al. 2022. Mol Cancer Res. 20:501. PubMed Xie J, et al. 2018. Oncol Lett. 16:157. PubMed Yamasaki S, et al. 2022. iScience. 25:103657. PubMed Hara M, et al. 2021. Materials (Basel). 14:. PubMed Yan K, et al. 2022. iScience. 25:104822. PubMed Cornelius C et al. 2016. EBioMedicine. 11:58-67 . PubMed Kim JH, et al. 2019. Mol Med. 25:33. PubMed Godbersen C, et al. 2017. Mol Cancer Ther. 16:1335. PubMed Shiraki N, et al. 2022. Stem Cell Reports. 17:221. PubMed Tritschler H, et al. 2021. Cells. 10:. PubMed Grayson KA, et al. 2021. PLoS One. 16:e0246733. PubMed Kotetsu Y, et al. 2021. Biomedicines. 9:. PubMed Luo Y, et al. 2021. Nat Commun. 12:3913. PubMed Hopf A, et al. 2022. Bioengineering (Basel). 9:. PubMed Wegner J, et al. 2021. Sci Rep. 11:14983. PubMed Okita R, et al. 2021. Thorac Cancer. 12:775. PubMed Hemkemeyer SA, et al. 2020. J Biol Chem. . PubMed Butler L, et al. 2011. J Virol. 85:7321. PubMed Ji S, et al. 2021. Clin Sci (Lond). 135:1127. PubMed Liisborg C, et al. 2021. Aging (Albany NY). 13:25763. PubMed Perera MR, et al. 2022. Int J Mol Sci. 23:. PubMed Roybal KT et al. 2016. Cell. 167(2):419-432 . PubMed Zou T, et al. 2019. Nat Commun. 10:1205. PubMed Hofmann L, et al. 2022. Front Med (Lausanne). 9:904295. PubMed Mikulkova Z, et al. 2021. Sci Rep. 11:322. PubMed Kang YH, et al. 2019. Nat Commun. 10:912. PubMed Palacios-Acedo AL, et al. 2021. Front Oncol. 11:704945. PubMed Kim N, et al. 2020. Cancers (Basel). 12:. PubMed Mercadal M, et al. 2020. Int J Mol Sci. 21:00. PubMed Correia DV, et al. 2011. Blood. 118:992. PubMed Deng Z, et al. 2021. Mol Med Rep. :23. PubMed Liu Z, et al. 2021. Oncogene. 40:6273. PubMed Fujiwara K, et al. 2020. Cancers (Basel). 12:. PubMed Patel C, et al. 2022. Curr Protoc. 2:e400. PubMed Loo Yau H, et al. 2021. STAR Protocols. 2(2):100549. PubMed Sharei A, et al. 2015. PLoS One. 10:118803. PubMed Julian Buchrieser, William James, Michael D. Moore 2017. Stem Cell Reports. 8(2):334-345. PubMed Zhu ZC, et al. 2019. Cell Death Dis. 10:118. PubMed Shiba T, et al. 2019. J Allergy Clin Immunol. 144:1438. PubMed Lindström A, et al. 2017. Oncotarget. 8:51370. PubMed Anderson NR, et al. 2019. Cell Adh Migr. 13:163. PubMed Hu W, et al. 2022. Mater Today Bio. 14:100224. PubMed
RRID: AB_2888687 (BioLegend Cat. No. 400119) AB_2888687 (BioLegend Cat. No. 400120)
Gene ID: NA
Clone: MOPC-21
Regulatory Status: RUO
Isotype: Mouse IgG1, κ