Biolegend, 400150, PerCP/Cyanine5.5 Mouse IgG1, κ Isotype Ctrl Antibody, 100tests

The MOPC-21 immunoglobulin has unknown specificity. The isotype of this antibody is mouse IgG1, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
100tests
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The immunoglobulin was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The IgG1, κ isotype control solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this mouse IgG1, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your primary antibody. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA)
Application References(PubMed link indicates BioLegend citation): Carlsten M, et al. 2007. Cancer Res. 67:1317. PubMed Smed-Sörensen A, et al. 2008. Blood 111:5037. PubMed (FA) Bunesmann MM, et al. 2011. Am. J. Respir. Cell. Mol. Biol. Epub. PubMed Matsuyama T, et al. 2005. Infect. Immun. 73:1044. (IF) Correia DV, et al. 2011. Blood 118:992. (FC) PubMed Lian IA, et al. 2011. Placenta. 32:823. PubMed Bufe B, et al. 20015. J Biol Chem. 290:7369. PubMed
Product Citations: Hu X, et al. 2023. Nat Commun. 14:2020. PubMed Gong YY, et al. 2022. Cancer Res Commun. 2:842. PubMed Market M, et al. 2022. Int J Mol Sci. 23: . PubMed Ding L, et al. 2023. J Clin Invest. 133: . PubMed Diessner J, et al. 2014. Cell Death Dis. 5:1149. PubMed Jacob A, et al. 2020. Nature Protocols. 14(12):3303-3332.. PubMed Alysandratos KD, et al. 2021. Cell Reports. 36(9):109636. PubMed Kuen J, et al. 2017. PLoS One. 10.1371/journal.pone.0182039. PubMed Queckborner S, et al. 2020. Stem Cell Res Ther. 11:15. PubMed Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed Murata K, et al. 2019. Oncol Lett. 17:5830. PubMed Market M, et al. 2020. Front Immunol. 1.127083333. PubMed Zhu Y, et al. 2012. PLoS One. 7:e44131. PubMed Flieswasser T, et al. 2020. Cells. 9:00. PubMed Liang Y, et al. 2019. J Allergy Clin Immunol. 144:1025. PubMed Grandclaudon M, et al. 2019. Cell. 179:432. PubMed Shrestha P, et al. 2021. PLoS Pathog. 17:e1009091. PubMed Hubbard JJ, et al. 2020. J Exp Med. :217. PubMed Wang X, et al. 2021. Front Endocrinol (Lausanne). 12:666195. PubMed McCauley KB, et al. 2018. Curr Protoc Stem Cell Biol. 45:e51. PubMed Mendoza-Cabrera MI, et al. 2019. Innate Immun. :1753425919864658. PubMed Luo Y, et al. 2021. Nat Commun. 12:3913. PubMed Still C 2nd, et al. 2021. Cell Reports Medicine. 2(7):100343. PubMed Shopova IA, et al. 2020. MBio. 11:. PubMed Suzuki S, et al. 2021. STAR Protocols. 2:100683. PubMed Mikulkova Z, et al. 2021. Sci Rep. 11:322. PubMed Hawkins FJ, et al. 2020. Cell Stem Cell. 28(1):79-95.e8. PubMed Riegel K, et al. 2021. Cell Death Dis. 12:969. PubMed Elgamal S, et al. 2021. JCI Insight. 6:. PubMed Yang C, et al. 2019. J Transl Med. 1.004166667. PubMed Hong Y, et al. 2019. J Extracell Vesicles. 8:1670893. PubMed
RRID: AB_893680 (BioLegend Cat. No. 400149) AB_893664 (BioLegend Cat. No. 400150)
Gene ID: NA
Clone: MOPC-21
Regulatory Status: RUO
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.