Biolegend, 400320, APC Mouse IgG2b, κ Isotype Ctrl Antibody, 100μg

The MPC-11 immunoglobulin has unknown specificity. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
100μg
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).µg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The immunoglobulin was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: µg sizes: 0.2 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this mouse IgG2b, ? isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your primary antibody. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA).
Application References(PubMed link indicates BioLegend citation): Smed-Sörensen A, et al. 2008. Blood 111:5037. (FA) PubMed Podolin PL, et al. 2008. J. Immunol. 180:7989. (FC) PubMed
Product Citations: Gao Y, et al. 2016. Nat Methods. 10.1038/nmeth.4042. PubMed Hu X, et al. 2023. Nat Biotechnol. . PubMed Miliotis C, et al. 2021. Cancer Lett. 518:115. PubMed Wang Q, et al. 2022. Sci Adv. 8:eabq4722. PubMed Pan R, et al. 2022. Cell. 185:1521. PubMed Cao B, et al. 2022. Nat Commun. 13:6203. PubMed Mota STS, et al. 2019. Int J Mol Sci. 20. PubMed Fu Y, et al. 2020. Sci Rep. 10:9027. PubMed Schneider T, et al. 2015. Glycobiology. 25: 812-824. PubMed Chen LM, et al. 2021. Biosci Rep. 41:. PubMed Wolpert F, et al. 2013. Neuro Oncology. 16:382. PubMed Jafari M, et al. 2014. J Virol. 88:5062. PubMed Zhu Y, et al. 2019. Cell Stem Cell. 25:542. PubMed Foight GW, et al. 2019. Nat Biotechnol. 37:1209. PubMed Su S et al. 2018. Cell. 175(2):442-457 . PubMed Arlt A, et al. 2020. Mol Oncol. 14:571. PubMed Yang WH, et al. 2018. Cancer Res. 78:3761. PubMed Chen LM, et al. 2021. Biosci Rep. Online ahead of print. PubMed Rotolo A et al. 2018. Cancer cell. 34(4):596-610 . PubMed Luna-Yolba R, et al. 2021. Cancers (Basel). 13: . PubMed Hassounah NB, et al. 2019. Cancer Immunol Immunother. 68:407. PubMed
RRID: AB_326500 (BioLegend Cat. No. 400319) AB_326500 (BioLegend Cat. No. 400321) AB_326500 (BioLegend Cat. No. 400320) AB_326500 (BioLegend Cat. No. 400322)
Gene ID: NA
Clone: MPC-11
Regulatory Status: RUO
Isotype: Mouse IgG2b, κ