Biolegend, 400329, Alexa Fluor® 488 Mouse IgG2b, κ Isotype Ctrl Antibody, 100tests

The MPC-11 immunoglobulin has unknown specificity. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
100tests
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this mouse IgG2b, ? isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your primary antibody. Use our Concentration Lookup tool to find the exact concentrations of your lots of product. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA).
Application References(PubMed link indicates BioLegend citation): Smed-Sörensen A, et al. 2008. Blood 111:5037. (FA) PubMed Podolin PL, et al. 2008. J. Immunol. 180:7989. (FC) PubMed
Product Citations: Pannu J, et al. 2015. PLoS One. 10:117304. PubMed Li Q, et al. 2013. Acta Biochim Biophys Sin. 45:593. PubMed Podolin P, et al. 2008. J Immunol. 180:7989. PubMed Grant R, et al. 2021. Methods Protoc. 4:. PubMed Jeong HO, et al. 2022. iScience. 25:105358. PubMed
RRID: AB_389318 (BioLegend Cat. No. 400329)
Gene ID: NA
Clone: MPC-11
Regulatory Status: RUO
Isotype: Mouse IgG2b, κ