Biolegend, 400432, Ultra-LEAF™ Purified Rat IgG1, κ Isotype Ctrl Antibody, 1mg

The isotype of RTK2071 immunoglobulin is rat IgG1, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
1mg
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Trinitrophenol + KLH
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality tested ICFC, ICC, IHC, WB, IP, FA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this rat IgG1, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.
Application Notes: The RTK2071 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 400414) as negative control1. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 400432) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).
Application References(PubMed link indicates BioLegend citation): Riemann M, et al. 2005. J. Immunol. 175:3560. PubMed Wondimu Z, et al. 2010. Am. J. Pathol. 177:2334. PubMed
Product Citations: Yamamoto A, et al. 2021. FASEB J. 35:e21158. PubMed Cui P, et al. 2023. J Neuroinflammation. 20:63. PubMed Mabire M, et al. 2023. Nat Commun. 14:1830. PubMed Cheon SY, et al. 2022. J Invest Dermatol. 2668:142. PubMed Jou E, et al. 2022. Sci Immunol. 7:eabn0175. PubMed Parashar K, et al. 2020. Immun Inflamm Dis. 0.696527778. PubMed Zhao K, et al. 2022. Adv Sci (Weinh). 9:e2103838. PubMed Nie X et al. 2018. Neuron. 99(3):464-479 . PubMed Iizasa E, et al. 2021. Nat Commun. 12:2299. PubMed Mock JR, et al. 2020. Am J Respir Cell Mol Biol. 63:464. PubMed Jung H, et al. 2019. Autophagy. 15:1990. PubMed Shute A, et al. 2021. Microbiome. 9:186. PubMed Izumi G, et al. 2021. Nat Commun. 12:5029. PubMed Yao M, et al. 2022. Dis Markers. 2022:4253566. PubMed Zhang Z, et al. 2021. Front Immunol. 12:699478. PubMed Murdock B, et al. 2014. J Immunol. 193:4107. PubMed
RRID: AB_11150233 (BioLegend Cat. No. 400431) AB_11150772 (BioLegend Cat. No. 400432) AB_11150772 (BioLegend Cat. No. 400457) AB_11150772 (BioLegend Cat. No. 400458) AB_11150772 (BioLegend Cat. No. 400461) AB_11150772 (BioLegend Cat. No. 400462)
Gene ID: NA
Clone: RTK2071
Regulatory Status: RUO
Isotype: Rat IgG1, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.