Biolegend, 400504, Biotin Rat IgG2a, κ Isotype Ctrl Antibody, 200μg

The RTK2758 immunoglobulin reacts with KLH. The isotype of this antibody is rat IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
200μg
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Trinitrophenol + KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The immunoglobulin was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest.
Application Notes: Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), and Functional Assay (FA).
Application References(PubMed link indicates BioLegend citation): Nishimoto H, et al. 2005. Blood 106:4241. Seach N, et al. 2008. J. Immunol. 180:5384. PubMed Jiang P, et al. 1999. J. Biol. Chem. 274:559. (FA)
Product Citations: Monslow J, et al. 2020. Am J Pathol. 1118:190. PubMed Olesen L, et al. 2017. Neurobiol Dis. 10.1016/j.nbd.2017.04.021. PubMed Sawanobori Y, et al. 2021. Histochem Cell Biol. 156:133. PubMed Baker R, et al. 2008. J Autoimmun. 31:385. PubMed Ogita T, et al. 2020. Frontiers in Immunology. 0.721527778. PubMed Hasegawa T, et al. 2020. Sci Rep. 10:13480. PubMed Cianciaruso C, et al. 2020. Cell Reports. 27(10):3062-3080.e11.. PubMed Shimba A et al. 2018. Immunity. 48(2):286-298 . PubMed Kurashima Y, et al. 2021. Nat Commun. 12:1067. PubMed
RRID: AB_2783537 (BioLegend Cat. No. 400503) AB_2783537 (BioLegend Cat. No. 400504)
Gene ID: NA
Clone: RTK2758
Regulatory Status: RUO
Isotype: Rat IgG2a, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.