Biolegend, 400523, APC/Cyanine7 Rat IgG2a, κ Isotype Ctrl Antibody, 25μg

The RTK2758 immunoglobulin reacts with KLH. The isotype of this antibody is rat IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
25μg
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Trinitrophenol + KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The immunoglobulin was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), and Functional Assay (FA).
Application References(PubMed link indicates BioLegend citation): Nishimoto H, et al. 2005. Blood 106:4241. Seach N, et al. 2008. J. Immunol. 180:5384. PubMed Jiang P, et al. 1999. J. Biol. Chem. 274:559. (FA)
Product Citations: Urata S, et al. 2023. Microbiol Immunol. 67:105. PubMed Machata S, et al. 2021. Front Immunol. 11:565869. PubMed Fu B et al. 2017. Immunity. 47(6):1100-1113 . PubMed Chen X, et al. 2021. Theranostics. 11:4655. PubMed Joseph R, et al. 2021. Br J Cancer. 125:176. PubMed He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed Domingos-Pereira S, et al. 2019. Cancer Immunol Res. 7:621. PubMed Tomimatsu M, et al. 2022. Sci Rep. 12:16656. PubMed Gross KM, et al. 2019. Cell Rep. 28:394. PubMed
RRID: AB_2923252 (BioLegend Cat. No. 400523) AB_2923252 (BioLegend Cat. No. 400524)
Gene ID: NA
Clone: RTK2758
Regulatory Status: RUO
Isotype: Rat IgG2a, κ