Biolegend, 400526, Alexa Fluor® 647 Rat IgG2a, κ Isotype Ctrl Antibody, 100μg

The RTK2758 immunoglobulin reacts with KLH. The isotype of this antibody is rat IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
100μg
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Trinitrophenol + KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The IgG2a, κ isotype control solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this rat IgG2a, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), and Functional Assay (FA).
Application References(PubMed link indicates BioLegend citation): Nishimoto H, et al. 2005. Blood 106:4241. Seach N, et al. 2008. J. Immunol. 180:5384. PubMed Jiang P, et al. 1999. J. Biol. Chem. 274:559. (FA)
Product Citations: Mizutani T, et al. 2023. iScience. 26:106081. PubMed El-Naccache DW, et al. 2022. Cell Rep. 40:111150. PubMed Bouthelier A, et al. 2023. iScience. 26:105739. PubMed Gon Y, et al. 2023. PLoS One. 18:e0279389. PubMed Reticker-Flynn NE, et al. 2022. Cell. 185:1924. PubMed Merz SF, et al. 2019. Nat Commun. 10:2312. PubMed Davis F, et al. 2015. Proc Natl Acad Sci U S A. 112:5827. PubMed Haile S, et al. 2013. J Immunol. 191:2829. PubMed Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed Akram KM, et al. 2019. Nat Commun. 10:1178. PubMed Garcia N, et al. 2011. J Immunol. 187:2268. PubMed Viveiros A, et al. 2022. Cells. 11:. PubMed Gottfried-Blackmore A, et al. 2009. Proc Natl Acad Sci U S A. 106:20918. PubMed Guo P, et al. 2021. J Immunol. 207:408. PubMed Broderick L, et al. 2019. Nat Commun. 10:3644. PubMed Yang T, et al. 2022. Nat Commun. 13:4464. PubMed Niemi JP, et al. 2020. Methods Mol Biol. 2143:207. PubMed Bochenek MA, et al. 2018. Nat Biomed Eng. 2:810. PubMed Patras L, et al. 2020. Int J Mol Sci. 21:00. PubMed Huang S, et al. 2021. FEBS Lett. 595:2463. PubMed Tomita T, et al. 2021. Nat Commun. 12:3655. PubMed Gonzalez MM, et al. 2021. J Clin Invest. 131:. PubMed
RRID: AB_2864284 (BioLegend Cat. No. 400526)
Gene ID: NA
Clone: RTK2758
Regulatory Status: RUO
Isotype: Rat IgG2a, κ