Biolegend, 400565, Ultra-LEAF™ Purified Rat IgG2a, κ Isotype Ctrl Antibody, 5mg

The RTK2758 immunoglobulin reacts with KLH. The isotype of this antibody is rat IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
5mg
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Trinitrophenol + KLH
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality tested ICFC, WB, IP, ICC, IHC, FA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this rat IgG2a, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.
Application Notes: Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), and Functional Assay (FA).
Application References(PubMed link indicates BioLegend citation): Nishimoto H, et al. 2005. Blood 106:4241. Seach N, et al. 2008. J. Immunol. 180:5384. PubMed Jiang P, et al. 1999. J. Biol. Chem. 274:559. (FA)
Product Citations: Wang YC, et al. 2023. J Exp Med. 220:. PubMed Giraud J, et al. 2024. Cell Rep. 113773:43. PubMed Bishop EL, et al. 2022. Immunology. 166:299. PubMed Li Y, et al. 2023. Theranostics. 13:403. PubMed Pernet E, et al. 2023. Nature. 614:530. PubMed Shimizu K, et al. 2020. Molecular Cell. 77(5):937-950.e6.. PubMed Pilch Z, et al. 2018. Cancer Immunol Res. 6:930. PubMed Menzel L, et al. 2021. Cell Rep. 37:109878. PubMed Ma YV, et al. 2021. MAbs. 13:2003281. PubMed Handke J, et al. 2021. Front Cardiovasc Med. 8:682458. PubMed Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed Mbofung R, et al. 2017. Nat Commun. 10.1038/s41467-017-00449-z. PubMed Nabar NR, et al. 2022. Autophagy. 18:204. PubMed Bacigaluppi M, et al. 2016. J Neurosci. 36: 10529 - 10544. PubMed Vito A, et al. 2021. Commun Biol. 4:859. PubMed Kim AD, et al. 2021. Sci Rep. 11:24194. PubMed Gu C, et al. 2021. Front Immunol. 12:678036. PubMed Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed Kumagai S, et al. 2020. Immunity. 53(1):187-203.e8. PubMed Kugel CH, et al. 2018. Clin Cancer Res. 24:5347. PubMed Obert E, et al. 2022. Heliyon. 8:e11359. PubMed Zheng Z, et al. 2021. Nat Commun. 12:6202. PubMed Walker MT, et al. 2018. J Allergy Clin Immunol. 141:1711. PubMed Weckbach LT, et al. 2019. J Exp Med. 216:350. PubMed Bok I, et al. 2022. JID Innov. 2:100076. PubMed Verma M, et al. 2021. J Exp Med. 218:. PubMed Rivera CA, et al. 2022. Immunity. 55:129. PubMed
RRID: AB_11148951 (BioLegend Cat. No. 400543) AB_11147167 (BioLegend Cat. No. 400544) AB_11147167 (BioLegend Cat. No. 400565) AB_11147167 (BioLegend Cat. No. 400566) AB_11147167 (BioLegend Cat. No. 400573) AB_11147167 (BioLegend Cat. No. 400574)
Gene ID: NA
Clone: RTK2758
Regulatory Status: RUO
Isotype: Rat IgG2a, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.