Biolegend, 400601, Purified Rat IgG2b, κ Isotype Ctrl Antibody, 50μg

The isotype of RTK4530 immunoglobulin is rat IgG2b, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
50μg
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Trinitrophenol + KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The immunoglobulin was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality testedICFC, ICC, IHC, IP, WB - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.
Application Notes: The RTK4530 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 400643, 400644, 400671, 400672, 400675, and 400676) as negative control.
Application References(PubMed link indicates BioLegend citation): Cervantes-Barragan L, et al. 2007. Blood 109:1131. Zeiser R, et al. 2007. Blood 109:2225. Sasaki K, et al. 2008. J. Immunol. 181:104. PubMed Duan J, et al. 2008. P. Natl. Acad. Sci. USA 105:5183. PubMed Yi H, et al. 2009. Blood 113:5819. PubMed Schafeer JS, et al. 2010. J. Leukocyte Biol. 87:301. PubMed Lei GS, et al. 2015. Infect Immun. 83:572. PubMed Richards J, et al. 2015. Mol Cell Cardiol. 79:21. PubMed
Product Citations: Moyat M, et al. 2015. Infect Immun . 83: 4217 - 4228. PubMed Liu HJ, et al. 2023. Nat Commun. 14:1214. PubMed Krovi SH, et al. 2019. Proc Natl Acad Sci U S A. 116:22252. PubMed Richards J, et al. 2015. J Mol Cell Cardiol. 79:21. PubMed Mukherjee D, et al. 2022. Nat Commun. 13:3747. PubMed Vackova J, et al. 2020. Int J Mol Sci. 21:00. PubMed Sasaki K, et al. 2008. J Immunol. 181:104. PubMed Ray HC, et al. 2020. Sci Rep. 10:15808. PubMed Matundan HH, et al. 2019. JCI Insight. 4:3. PubMed Yi H, et al. 2009. Blood. 113:5819. PubMed Steffen E, et al. 2020. Basic Res Cardiol. 18:115. PubMed Karhadkar TR, et al. 2021. PLoS One. 16:e0245924. PubMed Alexander KA, et al. 2019. Front Immunol. 10:377. PubMed Sasaki K, et al. 2007. Cancer Res. 67:6451. PubMed Lei G, et al. 2015. Infect Immun . 83:572. PubMed Coden ME, et al. 2020. J Immunol. 204:438. PubMed Atretkhany KN, et al. 2018. Proc Natl Acad Sci U S A. 115:13051. PubMed Yahata T, et al. 2019. Cancer Sci. 110:1279. PubMed Tanaka Y, et al. 2020. Sci Rep. 10:17284. PubMed Heng WS, et al. 2021. Lung Cancer. 154:13. PubMed Jiao J, et al. 2021. Basic Res Cardiol. 116:46. PubMed Xu L, et al. 2020. Nat Cell Biol. 1162:22. PubMed Saranchova I, et al. 2016. Sci Rep. 6: 30555. PubMed Duan J, et al. 2008. Proc Natl Acad Sci U S A. 105:5183. PubMed Lin D, et al. 2018. EMBO J. 37. PubMed Lin A, et al. 2022. Bioeng Transl Med. 7:e10314. PubMed Phan TS, et al. 2021. Sci Adv. 7:. PubMed Hwang BJ, et al. 2019. Oncogene. 38:7491. PubMed Prideaux M, et al. 2021. Sci Rep. 11:22593. PubMed Schaefer J, et al. 2009. J Leukoc Biol. 87:301. PubMed
RRID: AB_326545 (BioLegend Cat. No. 400601) AB_326546 (BioLegend Cat. No. 400602)
Gene ID: NA
Clone: RTK4530
Regulatory Status: RUO
Isotype: Rat IgG2b, κ