Biolegend, 400618, PE/Cyanine7 Rat IgG2b, κ Isotype Ctrl Antibody, 100μg

The isotype of RTK4530 immunoglobulin is rat IgG2b, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
100μg
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Trinitrophenol + KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The immunoglobulin was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The RTK4530 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 400643, 400644, 400671, 400672, 400675, and 400676) as negative control.
Application References(PubMed link indicates BioLegend citation): Cervantes-Barragan L, et al. 2007. Blood 109:1131. Zeiser R, et al. 2007. Blood 109:2225. Sasaki K, et al. 2008. J. Immunol. 181:104. PubMed Duan J, et al. 2008. P. Natl. Acad. Sci. USA 105:5183. PubMed Yi H, et al. 2009. Blood 113:5819. PubMed Schafeer JS, et al. 2010. J. Leukocyte Biol. 87:301. PubMed Lei GS, et al. 2015. Infect Immun. 83:572. PubMed Richards J, et al. 2015. Mol Cell Cardiol. 79:21. PubMed
Product Citations: Ho J, et al. 2016. PLoS Biol. 14:e2000117. PubMed White Z, et al. 2022. Blood Cells Mol Dis. 97:102688. PubMed Dona MSI, et al. 2023. Basic Res Cardiol. 118:11. PubMed Merah-Mourah F, et al. 2021. Curr Protoc. 1:e229. PubMed Tanner MA, et al. 2021. Am J Physiol Heart Circ Physiol. 321:H633. PubMed Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed Fujita Y et al. 2018. Cell reports. 24(12):3296-3311 . PubMed Chamoto K, et al. 2012. Am J Respir Cell Mol Biol. 46:283. PubMed Zhu H, et al. 2021. Cell Metabolism. :. PubMed Ying Zhang et al. 2017. Cancer cell. 32(3):377-391 . PubMed Kumagai S, et al. 2020. Immunity. 53(1):187-203.e8. PubMed Nakornpakdee Y, et al. 2018. Asian Pac J Allergy Immunol. 36:265. PubMed Belogortseva N, et al. 2017. PLoS One.. 10.1371/journal.pone.0182825. PubMed Zhou C, et al. 2020. Gut Microbes. 12:1782156. PubMed Chithanathan K, et al. 2022. Biology (Basel). 11:. PubMed
RRID: AB_326559 (BioLegend Cat. No. 400617) AB_326560 (BioLegend Cat. No. 400618)
Gene ID: NA
Clone: RTK4530
Regulatory Status: RUO
Isotype: Rat IgG2b, κ