Biolegend, 400627, Pacific Blue™ Rat IgG2b, κ Isotype Ctrl Antibody, 100μg

The isotype of RTK4530 immunoglobulin is rat IgG2b, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
100μg
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Trinitrophenol + KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The immunoglobulin was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: This reagent is developed for immunofluorescent staining for flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest. It is highly recommended that the reagent be titrated for optimal performance for each application. * Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Violet Laser (405 nm)
Application Notes: The RTK4530 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 400643, 400644, 400671, 400672, 400675, and 400676) as negative control.
Application References(PubMed link indicates BioLegend citation): Cervantes-Barragan L, et al. 2007. Blood 109:1131. Zeiser R, et al. 2007. Blood 109:2225. Sasaki K, et al. 2008. J. Immunol. 181:104. PubMed Duan J, et al. 2008. P. Natl. Acad. Sci. USA 105:5183. PubMed Yi H, et al. 2009. Blood 113:5819. PubMed Schafeer JS, et al. 2010. J. Leukocyte Biol. 87:301. PubMed Lei GS, et al. 2015. Infect Immun. 83:572. PubMed Richards J, et al. 2015. Mol Cell Cardiol. 79:21. PubMed
Product Citations: Jauch-Speer SL, et al. 2022. Elife. 11: . PubMed Gerwing M, et al. 2020. Mol Imaging Biol. 1.959027778. PubMed Girousse A et al. 2019. Cell Rep. 27(2):323-333 . PubMed Fujiwara Y, et al. 2021. Pancreas. 50:405. PubMed Mrdjen D et al. 2018. Immunity. 48(2):380-395 . PubMed Kooreman NG et al. 2018. Cell stem cell. 22(4):501-513 . PubMed Jamali A, et al. 2020. Cell Reports. 32(9):108099. PubMed Iwata TN, et al. 2018. Mol Cancer Ther. 17:1494. PubMed Zhang H et al. 2018. Cell. 175(5):1244-1258 . PubMed Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
RRID: AB_493561 (BioLegend Cat. No. 400627)
Gene ID: NA
Clone: RTK4530
Regulatory Status: RUO
Isotype: Rat IgG2b, κ