Biolegend, 401204, Biotin Mouse IgG2b, κ Isotype Ctrl Antibody, 200μg

The isotype of MG2b-57 immunoglobulin is mouse IgG2b,κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
200μg
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Trinitrophenol + KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest.
Application Notes: Additional reported applications (for the relevant formats) include: Intracellular flow cytometry, Western blotting, immunoprecipitation, immunocytochemistry, immunohistochemistry, functional assay. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 401215-401220) as negative control.
Application References(PubMed link indicates BioLegend citation): Magatti M, et al. 2008. Stem Cells 26:182. (FC) PubMed
Product Citations: Ying SQ, et al. 2024. J Nanobiotechnology. 521:22. PubMed
RRID: AB_326633 (BioLegend Cat. No. 401203) AB_326633 (BioLegend Cat. No. 401204)
Gene ID: NA
Clone: MG2b-57
Regulatory Status: RUO
Isotype: Mouse IgG2b, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.