Biolegend, 401602, Purified Mouse IgM, κ Isotype Ctrl Antibody, 500μg

The isotype of MM-30 immunoglobulin is mouse IgM, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
500μg
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Trinitrophenol + KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The immunoglobulin solution should be stored undiluted between 2°C and 8°C
Application: FC, ICFC - Quality tested WB, IP, ICC, IHC, FA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 85% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting, immunoprecipitation, immunocytochemistry, immunohistochemistry, and functional assay. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 401604) as negative control.
Application References(PubMed link indicates BioLegend citation): Ameres S, et al. 2014. J Immunol. 192:5894. PubMed
Product Citations: Deng S, et al. 2023. Exp Neurol. 367:114451. PubMed Ameres S, et al. 2014. J Immunol. 192:5894. PubMed Gawish R, et al. 2022. Elife. 11:. PubMed Winkler A, et al. 2021. J Clin Invest. 131:. PubMed van der Valk F, et al. 2016. Circulation. 134: 611 - 624. PubMed Wallis J, et al. 2020. Eur J Pharm Sci. 152:105456. PubMed Rudnick RB, et al. 2018. J Immunol. 200:2280. PubMed Li H, et al. 2020. Front Immunol. 11:1777. PubMed Kobayashi K, et al. 2018. J Virol. 92:e00681. PubMed Zhang T, et al. 2020. PLoS One. 15:e0239601. PubMed Ulndreaj A, et al. 2021. Eneuro. 8:. PubMed Wülfing C, et al. 2018. Immun Inflamm Dis. 0.495833333. PubMed
RRID: AB_2935847 (BioLegend Cat. No. 401601) AB_2935847 (BioLegend Cat. No. 401602)
Gene ID: NA
Clone: MM-30
Regulatory Status: RUO
Isotype: Mouse IgM, κ