Biolegend, 401605, FITC Mouse IgM, κ Isotype Ctrl Antibody, 25tests

The isotype of MM-30 immunoglobulin is mouse IgM, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
25tests
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Trinitrophenol + KLH
Formulation: test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The immunoglobulin was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: µg sizes: 0.5 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this mouse IgM, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your primary antibody. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting, immunoprecipitation, immunocytochemistry, immunohistochemistry, and functional assay. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 401604) as negative control.
Application References(PubMed link indicates BioLegend citation): Ameres S, et al. 2014. J Immunol. 192:5894. PubMed
Product Citations: Garcia-Larragoiti N, et al. 2021. J Thromb Haemost. 19:2572. PubMed Cano-Mendez A, et al. 2022. Int J Mol Sci. 23: . PubMed Zhang HC, et al. 2018. Int J Mol Med. 41:13. PubMed
RRID: AB_389346 (BioLegend Cat. No. 401605) AB_389346 (BioLegend Cat. No. 401607) AB_389346 (BioLegend Cat. No. 401606)
Gene ID: NA
Clone: MM-30
Regulatory Status: RUO
Isotype: Mouse IgM, κ