Biolegend, 402404, Spark NIR™ 685 Mouse IgG1, κ Isotype Ctrl Antibody, 100tests

The MOPC-21 immunoglobulin has unknown specificity. The isotype of this antibody is mouse IgG1, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
100tests
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark NIR™ 685 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Spark NIR™ 685 has a maximum excitation of 665 nm and a maximum emission of 685 nm.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA)
Application References(PubMed link indicates BioLegend citation): Carlsten M, et al. 2007. Cancer Res. 67:1317. PubMed Smed-Sörensen A, et al. 2008. Blood 111:5037. PubMed (FA) Bunesmann MM, et al. 2011. Am. J. Respir. Cell. Mol. Biol. Epub. PubMed Matsuyama T, et al. 2005. Infect. Immun. 73:1044. (IF) Correia DV, et al. 2011. Blood 118:992. (FC) PubMed Lian IA, et al. 2011. Placenta. 32:823. PubMed Bufe B, et al. 20015. J Biol Chem. 290:7369. PubMed
RRID: AB_3097092 (BioLegend Cat. No. 402403) AB_3097092 (BioLegend Cat. No. 402404)
Gene ID: NA
Clone: MOPC-21
Regulatory Status: RUO
Isotype: Mouse IgG1, κ