Biolegend, 405150, Purified Streptavidin, 1mg

Streptavidin is a 52 kD tetrameric protein obtained from Streptomyces avidinii . It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10 -14 mol/L. It is used in a wide range of applications including ELISA, flow cytometry, molecular biology, and bionanotechnology.
1mg
Formulation: Each vial contains Streptavidin lyophilized in sodium chloride buffer.
Preparation: Reconstitute the content of the vial in distilled water to the desired concentration.
Storage & Handling: For long-term storage, store the lyophilized streptavidin at -20°C or -70°C. After reconstitution, store the solution between 2°C and 8°C for up to 2 weeks, or store in aliquots at -20°C for up to 6 months.
Application: FC - Quality tested ELISA Capture, ELISA Detection, ICC, IHC-F, IHC-P, IP, WB - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Nicosia M, et al. 2023. J Leukoc Biol. 544:113. PubMed Rajagopalan A, et al. 2021. Cell Rep Methods. 1:. PubMed Urlaub D, et al. 2020. Curr Protoc Immunol. 131:e111. PubMed Ridge LA, et al. 2021. Cell Rep. 36:109610. PubMed
Structure: 52 kD tetrameric protein
Distribution: Streptavidin binds to biotin with high affinity
Ligand/Receptor: Biotin
Antigen References: 1. Dundas CM, et al. 2013. Appl. Microbiol. Biotechnol. 97:9343. 2. Zhao X, et al. 2013. J. Anal. Methods Chem. 2013:581093. 3. Kaplan DL, et al. 1999. Biomol. Eng. 16:135. 4. Wilbur DS, et al. 1999. Biomol. Eng. 16:113. 5. Sano T, et al. 1998. J. Chromatogr. B. Biomed. Sci. Appl. 715:85.
Gene ID: NA
UniProt: View information about Biotin on UniProt.org
Regulatory Status: RUO
Other Names: Streptavidin-PURE, SAV-PU, SAV