Biolegend, 405301, Purified Goat anti-mouse IgG (minimal x-reactivity) Antibody, 500μg

This polyclonal goat anti-mouse IgG antibody reacts with the heavy chains of mouse IgG and with the light (kappa and lambda) chains common to most mouse immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and rabbit immunoglobulins, but it may have minimal cross-reactivity with other subclasses of mouse immunoglobulins.
500μg
Verified Reactivity: Mouse
Antibody Type: Polyclonal
Host Species: Goat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ELISA - Quality tested FC, WB, IHC
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture, the suggested use of this reagent is ≤ 2.0 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This polyclonal goat anti-mouse IgG antibody is useful for capture or detection of mouse IgG in ELISA.
Application References(PubMed link indicates BioLegend citation): Scotta C, et al. 2008. J Immunol. 181:1025-33. PubMed Gao X, et al. 2014. Cell Mol Neurobiol. 34:257-68 (ICC) Winters T, et al. 2014. EMBO J. 33:1256-70.
Product Citations: Tocheva AS, et al. 2020. Curr Protoc Immunol. 130:e103. PubMed MacLean AJ, et al. 2022. Immunity. 55:718. PubMed Galindo-Albarrán A, et al. 2016. Cell Rep. 17:2151-2160. PubMed Rivers E, et al. 2020. Elife. 9:00. PubMed özdilek A, et al. 2020. Proc Natl Acad Sci U S A. 117:1280. PubMed Sánchez–Villanueva JA, et al. 2019. PLoS One. 14:e0226388. PubMed Scottà C, et al. 2008. J Immunol. 181:1025. PubMed Cheung KCP, et al. 2020. Nat Commun. 2.954861111. PubMed
RRID: AB_315005 (BioLegend Cat. No. 405301)
Distribution: B cells
Gene ID: 16059
UniProt: View information about IgG on UniProt.org
Clone: Poly4053
Regulatory Status: RUO
Isotype: Goat Polyclonal IgG