Biolegend, 405312, DyLight™ 649 Goat anti-mouse IgG (minimal x-reactivity) Antibody, 100μg

This polyclonal goat anti-mouse IgG antibody reacts with the heavy chains of mouse IgG and with the light (kappa and lambda) chains common to most mouse immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and rabbit immunoglobulins, but it may have minimal cross-reactivity with other subclasses of mouse immunoglobulins.
100μg
Verified Reactivity: Mouse
Antibody Type: Polyclonal
Host Species: Goat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with DyLight™ 649 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * DyLight™ 649 has a maximum absorption of 655nm and a maximum emission of 670nm (similar to Alexa Fluor® 647 and APC).
Application Notes: This polyclonal goat anti-mouse IgG antibody is useful for capture or detection of mouse IgG in ELISA.
Application References(PubMed link indicates BioLegend citation): Scotta C, et al. 2008. J Immunol. 181:1025-33. PubMed Gao X, et al. 2014. Cell Mol Neurobiol. 34:257-68 (ICC) Winters T, et al. 2014. EMBO J. 33:1256-70.
Product Citations: Segawa S, et al. 2014. Rheumatology. 53:2259. PubMed Parvatiyar K, et al. 2018. Nat Commun. 9:2770. PubMed Biering SB et al. 2017. Cell host & microbe. 22(1):74-85 . PubMed Dai B, et al. 2022. Theranostics. 12:7603. PubMed Zhang T, et al. 2012. J Immunol. 189:2290. PubMed Locci M, et al. 2016. Nat Immunol. 17: 976-984. PubMed Branch M, et al. 2012. Invest Ophthalmol Vis Sci . 53:5109. PubMed Chen Y, et al. 2016. PLoS One. 11: 0149485. PubMed Yin S, et al. 2015. Sci Rep. 5: 14432. PubMed Chen Y, et al. 2014. Blood. 124:936. PubMed Sepahi A, et al. 2016. Sci Rep. 6:39054. PubMed Moritsch S, et al. 2022. Oncoimmunology. 11:2127271. PubMed
RRID: AB_1575128 (BioLegend Cat. No. 405312)
Distribution: B cells
Gene ID: 16059
UniProt: View information about IgG on UniProt.org
Clone: Poly4053
Regulatory Status: RUO
Isotype: Goat Polyclonal IgG