Biolegend, 405420, Alexa Fluor® 555 Goat anti-rat IgG (minimal x-reactivity) Antibody, 100μg

Goat anti-rat IgG antibody reacts primarily with the heavy chains of rat IgG, but also partially binds to the light chains common to most rat immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rabbit, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other immunoglobulins from other species.
100μg
Verified Reactivity: Rat
Antibody Type: Polyclonal
Host Species: Goat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 555 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICC - Quality tested IHC-F - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/mL is recommended. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 555 has an excitation maximum of 555 nm, and a maximum emission of 565 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: This goat anti-rat IgG antibody is useful for direct immunofluorescent staining or as a second step reagent for indirect immunofluorescent staining when used in conjunction with primary antibodies.
Application References(PubMed link indicates BioLegend citation): Zornetzer GA, et al. 2010. J. Virol. 84:11297. PubMed Frank NY, et al. 2011. Cancer Res. 71:1474. (IHC) PubMed Baba T, et al. 2020. eLife. 9:e54257. (IHC-P)
Product Citations: Wang JX, et al. 2023. JCI Insight. 8:. PubMed Zhang Z, et al. 2022. Cancer Gene Ther. 29:722. PubMed Hsiao HW, et al. 2023. Biomolecules. 13: . PubMed Challa DK, et al. 2019. MAbs. 11:848. PubMed Luo L, et al. 2021. J Neuroinflammation. 18:27. PubMed Kurashima Y, et al. 2021. Nat Commun. 12:1067. PubMed
RRID: AB_2563180 (BioLegend Cat. No. 405420)
Gene ID: 295279
UniProt: View information about IgG on UniProt.org
Clone: Poly4054
Regulatory Status: RUO
Isotype: Goat Polyclonal IgG