Biolegend, 405712, Pacific Blue™ anti-mouse IgD Antibody, 100μg

Surface IgD is an important B cell differentiation marker.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Violet Laser (405 nm)
Application Notes: The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2,3, and spatial biology (IBEX)10,11.
Application References(PubMed link indicates BioLegend citation): Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC) Weih D, et al. 2001. J. Immunol. 167:1909. (IHC) Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC) Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC) Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed Park SY, et al. 2013. J. Immunol. 190:1094. PubMed Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Lombard-Vadnais F, et al. 2023. iScience. 26:105852. PubMed Kodali S, et al. 2022. J Immunol. 208:1085. PubMed Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed Shikatani EA et al. 2019. Cell Rep. 27(8):2304-2312 . PubMed Kim Y, et al. 2015. PLoS One. 10:120294. PubMed Mathew NR, et al. 2021. Cell Reports. 35(12):109286. PubMed Dave K et al. 2017. eLife. 6 pii: e23382. PubMed Holodick N, et al. 2014. J Immunol. 192:2432. PubMed Muppidi J, et al. 2015. J Exp Med. 212: 2213 - 2222. PubMed McGuire A, et al. 2016. Nat Commun. 7:10618. PubMed Chen M, et al. 2014. PLoS One. 9:107505. PubMed Duan L, et al. 2021. Immunity. 54:2256. PubMed Ray A, et al. 2015. PLoS One. 10: 0137314. PubMed Qi T, et al. 2020. Cell Rep. 33:108452. PubMed Noviski M, et al. 2018. Elife. 7:e35074. PubMed Holodick N, et al. 2016. J Immunol. 196: 4348 - 4357. PubMed Olson WJ, et al. 2019. Cell Rep. 28:2878. PubMed Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed Clancy–Thompson E, et al. 2019. EMBO J. 38:e101260. PubMed Vijay R, et al. 2021. J Exp Med. 218:. PubMed Wright JA, et al. 2021. Front Immunol. 12:705307. PubMed Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed Vijay R, et al. 2020. Nat Immunol. 21:790. PubMed Ang QY, et al. 2020. Cell. 181(6):1263-1275.e16. PubMed
RRID: AB_1937245 (BioLegend Cat. No. 405711) AB_1937244 (BioLegend Cat. No. 405712)
Structure: Ig family
Distribution: B cells
Function: B cell differentiation
Cell Type: B cells
Biology Area: Immunology
Gene ID: 380797
UniProt: View information about IgD on UniProt.org
Clone: 11-26c.2a
Regulatory Status: RUO
Other Names: Immunoglobulin D
Isotype: Rat IgG2a, κ