Biolegend, 405720, PE/Cyanine7 anti-mouse IgD Antibody, 100μg

Surface IgD is an important B cell differentiation marker.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2,3, and spatial biology (IBEX)10,11.
Application References(PubMed link indicates BioLegend citation): Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC) Weih D, et al. 2001. J. Immunol. 167:1909. (IHC) Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC) Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC) Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed Park SY, et al. 2013. J. Immunol. 190:1094. PubMed Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Melzi E, et al. 2022. Immunity. 55:2168. PubMed Lee JH, et al. 2022. Cell Rep. 38:110485. PubMed Yang K, et al. 2022. Sci Immunol. 7:eabn2888. PubMed Grégoire C, et al. 2022. Immunity. 55:1216. PubMed Zhang J, et al. 2021. MedComm (Beijing). 2:256. PubMed Zhu X, et al. 2017. Arch Oral Biol. 10.1016/j.archoralbio.2017.03.010. PubMed Tu X, et al. 2022. Nat Commun. 13:6977. PubMed Bourque J, et al. 2021. Heliyon. 7:e08311. PubMed Alameh MG, et al. 2021. Immunity. 54:2877. PubMed Wang X, et al. 2021. EMBO J. 40:e105926. PubMed Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed Werner A, et al. 2021. iScience. 24:103076. PubMed Escolano A, et al. 2016. Cell. 166(6):1445-1458.e12. PubMed Lederer K, et al. 2020. Immunity. 53(6):1281-1295.e5. PubMed Stienne C, et al. 2022. Cell Rep. 38:110553. PubMed
RRID: AB_2561875 (BioLegend Cat. No. 405719) AB_2561876 (BioLegend Cat. No. 405720)
Structure: Ig family
Distribution: B cells
Function: B cell differentiation
Cell Type: B cells
Biology Area: Immunology
Gene ID: 380797
UniProt: View information about IgD on UniProt.org
Clone: 11-26c.2a
Regulatory Status: RUO
Other Names: Immunoglobulin D
Isotype: Rat IgG2a, κ