Biolegend, 406206, FITC anti-mouse IgM, 500μg

This monoclonal antibody was raised by immunization with C57BL/10 mouse splenocytes. It specifically reacts with IgM of Igh-C b and the related haplotypes (d and n), but not with the free μ heavy chain of IgM. Some cross-reaction with IgM of Igh-C e can be detected with this antibody.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: ELISA as capture antibody or detection antibody, depletion of lymphocytes.
Application References(PubMed link indicates BioLegend citation): Stall AM, et al. 1984. J. Immunol. 132:787. Velardi A, et al. 1984. J. Immunol. 133:2098. Duong BH, et al. 2010. J. Immunol. 184:4143. PubMed Aschermann S, et al. 2013. PNAS. 110:19042. PubMed
Product Citations: Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed Avery DT, et al. 2018. J Exp Med. 215:2073. PubMed Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed Aschermann S, et al. 2013. Proc Natl Acad Sci U S A. 110:19042. PubMed Duong B, et al. 2010. J Immunol. 184:4143. PubMed Yamada H, et al. 2018. J Immunol. 201:3492. PubMed Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed Wan X, Thomas J, Unanue E 2016. J Exp Med. 213: 967 - 978. PubMed
RRID: AB_315038 (BioLegend Cat. No. 406205) AB_315039 (BioLegend Cat. No. 406206)
Biology Area: Immunology
Gene ID: 16019
UniProt: View information about IgMb on UniProt.org
Clone: AF6-78
Regulatory Status: RUO
Other Names: Igh-6b
Isotype: Mouse (BALB/c) IgG1, κ