Product Description
This polyclonal donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.
100μg
Verified Reactivity: Rabbit
Antibody Type: Polyclonal
Host Species: Donkey
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICC - Quality tested FC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 2.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: This conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.
Product Citations: González-Casimiro CM, et al. 2022. Int J Mol Sci. 23:. PubMed Shao W, et al. 2023. Biomedicines. 11:. PubMed Amirova KM, et al. 2023. Int J Mol Sci. 24:. PubMed Nicholas D, et al. 2015. Mol Cell Proteomics. 14:15. PubMed Fisher E, et al. 2021. Front Immunol. 11:602254. PubMed Jarosch S, et al. 2022. STAR Protoc. 3:101374. PubMed Zhao Y, et al. 2021. Front Immunol. 12:665442. PubMed Wong C, et al. 2014. J Leukoc Biol. 95:631. PubMed Sun Z, et al. 2021. eLife. 10:00. PubMed Callahan SM, et al. 2021. Curr Protoc. 1:e294. PubMed Tang H, et al. 2017. J Immunol. 199:570. PubMed Callahan S, et al. 2020. Cell Microbiol. 22:e13210. PubMed Woo YD, et al. 2020. J Allergy Clin Immunol. . PubMed Jarosch S, et al. 2021. Cell Rep Methods. 1:100104. PubMed Yang J, et al. 2020. Nature. 586:572. PubMed Kishore M et al. 2017. Immunity. 47(5):875-889 . PubMed Cai Y, et al. 2019. Cell Rep. 27:3034. PubMed Shen C, et al. 2021. Front Immunol. 12:680055. PubMed Niemeyer BF, et al. 2021. Nano Sel. . PubMed Yang Y, et al. 2020. J Clin Invest. 130:5508. PubMed Shiozawa S, et al. 2022. iScience. 25:103537. PubMed Janczy J, et al. 2014. J Immunol. 193:5190. PubMed Daneshmandi S, et al. 2021. Cell Reports. 34(10):108831. PubMed Zeng G, et al. 2011. J Immunol. 187:190. PubMed Mohamadi F, et al. 2019. Biomed Rep. 10:183. PubMed Tittarelli A, et al. 2014. J Immunol. 192:1313. PubMed Ramos-Ramírez P, et al. 2021. Front Immunol. 12:677550. PubMed Che N, et al. 2021. Front Immunol. 12:626310. PubMed Ryzhov S, et al. 2012. J Pharmacol Exp Ther. 341:764. PubMed Liu F, et al. 2021. Front Microbiol. 12:692279. PubMed
RRID: AB_893531 (BioLegend Cat. No. 406403)
Gene ID: 100343867
UniProt: View information about Rabbit IgG on UniProt.org
Clone: Poly4064
Regulatory Status: RUO
Isotype: Donkey Polyclonal Ig
Order Guidelines
1. Price & Stock Available on Request. Click to send email to: service@iright.com
2. Please DO NOT make payment before confirmation.
3. Minimum order value of $1,000 USD required.
Collaboration
Tony Tang
Email: Tony.Tang@iright.com
Mobile/WhatsApp/Wechat: +86-17717886924