Biolegend, 406501, Purified anti-mouse IgM Antibody, 50μg

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on the majority of mature B cells.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The IgM antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested ELISA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The RMM-1 antibody reacts with both soluble and membrane immunoglobulin M in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. Some formats of the RMM-1 antibody may be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.
Application References(PubMed link indicates BioLegend citation): Tertilt C, et al. 2009. Infect. Immun. 77:3044. (ELISA) PubMed
Product Citations: Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed Wang W, et al. 2020. Cell Rep. 107936:32. PubMed Mouat IC, et al. 2021. Elife. 10:. PubMed Sun L, et al. 2021. Cell Reports. 35(9):109188. PubMed Plzakova L, et al. 2015. PLoS One. 10: 0132571. PubMed Qiu Z, et al. 2022. Cancer Discov. 12:502. PubMed Englezou PC, et al. 2018. Mol Ther Nucleic Acids. 12:118. PubMed Cole C, et al. 2018. Nucleic Acids Res. 46:e62. PubMed Lin J, et al. 2017. Nat Commun. . 10.1038/s41467-017-01477-5. PubMed Maity P, et al. 2015. Sci Signal. 8: ra93. PubMed Tertilt C, et al. 2009. Infect Immun. 77:3044. PubMed
RRID: AB_315051 (BioLegend Cat. No. 406501) AB_315052 (BioLegend Cat. No. 406502)
Structure: Ig family
Distribution: B cells
Function: B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type: B cells
Biology Area: Immunology
Gene ID: 16019
UniProt: View information about IgM on UniProt.org
Clone: RMM-1
Regulatory Status: RUO
Other Names: Immunoglobulin M
Isotype: Rat IgG2a, κ