Biolegend, 406505, FITC anti-mouse IgM Antibody, 50μg

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on the majority of mature B cells.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: The RMM-1 antibody reacts with both soluble and membrane immunoglobulin M in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. Some formats of the RMM-1 antibody may be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.
Application References(PubMed link indicates BioLegend citation): Tertilt C, et al. 2009. Infect. Immun. 77:3044. (ELISA) PubMed
Product Citations: Rydyznski CE et al. 2018. Cell reports. 24(13):3367-3373 . PubMed Hutter K, et al. 2022. Front Immunol. 13:967914. PubMed Yang K, et al. 2022. Sci Immunol. 7:eabn2888. PubMed Kodali S, et al. 2022. J Immunol. 208:1085. PubMed Ercoli G, et al. 2021. Front Immunol. 11:611661. PubMed Vijayan K, et al. 2021. Cell Reports. 36(5):109489. PubMed Reed S, et al. 2014. PLoS One. 9:112126. PubMed Kurkewich J, et al. 2016. PLoS One. 11: 0161468. PubMed Tang H, et al. 2022. iScience. 25:104209. PubMed Qi T, et al. 2020. Cell Rep. 33:108452. PubMed Hutter K, et al. 2020. FEBS J. . PubMed Wilder BK, et al. 2022. NPJ Vaccines. 7:58. PubMed Glaros V, et al. 2021. Immunity. 54:2005. PubMed Ercoli G, et al. 2022. Clin Transl Immunology. 11:e1366. PubMed Magri G et al. 2017. Immunity. 47(1):118-134 . PubMed Zhang H, et al. 2019. Mol Cell. 76:110. PubMed Jelinek D, et al. 2014. J Immunol. 192:3548. PubMed Shiozawa S, et al. 2022. iScience. 25:103537. PubMed Lehrke MJ, et al. 2021. Elife. 10:. PubMed Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed Adachi Y, et al. 2019. Nat Commun. 10:3883. PubMed Yin S et al. 2019. Cancer cell. 35(2):283-296 . PubMed Ghezraoui H, et al. 2018. Nature. 560:122. PubMed Kleiman E, et al. 2016. Proc Natl Acad Sci U S A. 113: E3911 - E3920. PubMed Carbonetti S et al. 2017. Journal of immunological methods. 448:66-73 . PubMed
RRID: AB_315055 (BioLegend Cat. No. 406505) AB_315056 (BioLegend Cat. No. 406506)
Structure: Ig family
Distribution: B cells
Function: B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type: B cells
Biology Area: Immunology
Gene ID: 16019
UniProt: View information about IgM on UniProt.org
Clone: RMM-1
Regulatory Status: RUO
Other Names: Immunoglobulin M
Isotype: Rat IgG2a, κ