Biolegend, 406513, PE/Cyanine7 anti-mouse IgM Antibody, 25μg

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on the majority of mature B cells.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The RMM-1 antibody reacts with both soluble and membrane immunoglobulin M in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. Some formats of the RMM-1 antibody may be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.
Application References(PubMed link indicates BioLegend citation): Tertilt C, et al. 2009. Infect. Immun. 77:3044. (ELISA) PubMed
Product Citations: Hong H, et al. 2022. STAR Protoc. 3:101526. PubMed Yazicioglu YF, et al. 2023. Nat Immunol. 24:991. PubMed Thomsen I, et al. 2021. J Immunol. Online ahead of print. PubMed Koenig A, et al. 2022. Front Immunol. 12:791100. PubMed MacLean AJ, et al. 2022. Immunity. 55:718. PubMed Erickson JJ, et al. 2022. Nature. 606:769. PubMed Blanco LP, et al. 2022. Arthritis Rheumatol. 74:1971. PubMed Urata S, et al. 2023. Microbiol Immunol. 67:105. PubMed Lombard-Vadnais F, et al. 2023. iScience. 26:105852. PubMed Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed Brestoff JR, et al. 2021. Cell Metab. 33:270. PubMed Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed Hung PJ, et al. 2018. Mol Cell. 71:332. PubMed Cunha P, et al. 2022. Front Vet Sci. 9:891893. PubMed Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed Fredriksson-Lidman K, et al. 2017. PLoS One. 10.1371/journal.pone.0185509. PubMed Sauma D, et al. 2017. Immunol Res. 10.1007/s12026-017-8936-9. PubMed Zhang H, et al. 2019. Mol Cell. 76:110. PubMed Li H, et al. 2020. Nature. 584:274. PubMed Jonas B, et al. 2016. PLoS One. 11: 0159189. PubMed Souza SP, et al. 2021. PLoS Pathog. 17:e1010081. PubMed Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed Ghezraoui H, et al. 2018. Nature. 560:122. PubMed Cantor DJ et al. 2019. Cell reports. 26(1):108-118 . PubMed Habets T, et al. 2016. PLoS One. 11: 0159515. PubMed Staffas A et al. 2018. Cell host & microbe. 23(4):447-457 . PubMed Schardt JS, et al. 2021. Sci Rep. 11:20738. PubMed Nagatake T, et al. 2019. Mucosal Immunol. 12:p1082. PubMed
RRID: AB_10640069 (BioLegend Cat. No. 406513) AB_10642031 (BioLegend Cat. No. 406514)
Structure: Ig family
Distribution: B cells
Function: B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type: B cells
Biology Area: Immunology
Gene ID: 16019
UniProt: View information about IgM on UniProt.org
Clone: RMM-1
Regulatory Status: RUO
Other Names: Immunoglobulin M
Isotype: Rat IgG2a, κ