Biolegend, 406518, Brilliant Violet 421™ anti-mouse IgM Antibody, 500microl

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on the majority of mature B cells.
500microl
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: µg size: 0.2 mg/mLµL sizes: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: The RMM-1 antibody reacts with both soluble and membrane immunoglobulin M in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. Some formats of the RMM-1 antibody may be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.
Application References(PubMed link indicates BioLegend citation): Tertilt C, et al. 2009. Infect. Immun. 77:3044. (ELISA) PubMed
Product Citations: Pani F, et al. 2021. Endocrinology. 162:. PubMed Stabel JR, et al. 2022. PLoS One. 17:e0278313. PubMed Kodali S, et al. 2022. J Immunol. 208:1085. PubMed Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed Shikatani EA et al. 2019. Cell Rep. 27(8):2304-2312 . PubMed Liang Q, et al. 2022. iScience. 25:104043. PubMed Geng T, et al. 2022. Methods Mol Biol. 2585:71. PubMed Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed Weber G, et al. 2014. J Exp Med. 211:1243. PubMed Zhu Y, et al. 2022. Clin Transl Med. 12:e887. PubMed Knizkova D, et al. 2022. Nat Immunol. 23:1644. PubMed Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed Ghosh D, et al. 2016. J Immunol. 197: 1788 - 1800. PubMed Yamada H, et al. 2018. J Immunol. 201:3492. PubMed Wilmore JR, et al. 2022. Front Immunol. 12:791095. PubMed Hong JP, et al. 2020. Cell Reports Medicine. 1(3):100035. PubMed Jing Y, et al. 2019. J Allergy Clin Immunol. 144:1377. PubMed Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed Spanier J, et al. 2016. Nat Commun. 7:11804. PubMed
RRID: AB_10899576 (BioLegend Cat. No. 406517) AB_2650930 (BioLegend Cat. No. 406532) AB_2561444 (BioLegend Cat. No. 406518)
Structure: Ig family
Distribution: B cells
Function: B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type: B cells
Biology Area: Immunology
Gene ID: 16019
UniProt: View information about IgM on UniProt.org
Clone: RMM-1
Regulatory Status: RUO
Other Names: Immunoglobulin M
Isotype: Rat IgG2a, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.