Biolegend, 406619, APC/Cyanine7 anti-mouse IgG1 Antibody, 25μg

The RMG1-1 monoclonal antibody reacts with immunoglobulin G1 (IgG1) in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RMG1-1 monoclonal antibody may be used as a primary or secondary reagent for ELISA or immunofluorescent analysis.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Rucci F, et al. 2010. P. Natl. Acad. Sci. USA 107:3024. (FC) PubMed Zhang JG, et al. 2011. Int. Immunopharmacol. 11:1685. PubMed
Product Citations: Cequier A, et al. 2024. Front Vet Sci. 1391872:11. PubMed Rodriguez AB, et al. 2021. Cell Reports. 36(3):109422. PubMed Choi J, et al. 2021. Nat Cell Biol. 23:953. PubMed Putz EJ, et al. 2019. J Dairy Sci. 102:9268. PubMed Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
RRID: AB_2571946 (BioLegend Cat. No. 406619) AB_2571947 (BioLegend Cat. No. 406620)
Gene ID: 16017
UniProt: View information about IgG1 on UniProt.org
Clone: RMG1-1
Regulatory Status: RUO
Other Names: Immunoglobulin G1
Isotype: Rat IgG