Biolegend, 406708, PE anti-mouse IgG2b Antibody, 100μg

The RMG2b-1 monoclonal antibody reacts with immunoglobulin G2b (IgG2b) in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. The RMG2b-1 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Kimura S, et al. 2020. Nat Commun. 0.620833333. PubMed Grégoire C, et al. 2022. Immunity. 55:1216. PubMed Jagodinsky JC, et al. 2022. J Immunother Cancer. 10:. PubMed Hung PJ, et al. 2018. Mol Cell. 71:332. PubMed Delgado‐Benito V et al. 2018. Molecular cell. 72(4):636-649 . PubMed Sundaravinayagam D et al. 2019. Cell Rep. 28(6):1389-1399 . PubMed Ma X, et al. 2020. Immunity. 53:1315. PubMed Souza SP, et al. 2021. PLoS Pathog. 17:e1010081. PubMed Vincendeau E, et al. 2022. Nat Commun. 13:3707. PubMed Clancy–Thompson E, et al. 2019. EMBO J. 38:e101260. PubMed Liang YH, et al. 2021. J Biomed Sci. 28:75. PubMed
RRID: AB_2563380 (BioLegend Cat. No. 406707) AB_2563381 (BioLegend Cat. No. 406708)
Gene ID: 16016
UniProt: View information about IgG2b on UniProt.org
Clone: RMG2b-1
Regulatory Status: RUO
Other Names: Immunoglobulin G2b
Isotype: Rat IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.