Biolegend, 406804, Biotin anti-mouse IgG3 Antibody, 500μg

The RMG3-1 monoclonal antibody reacts with immunoglobulin G3 (IgG3) in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RMG3-1 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: ELISA, FC
Recommended Usage: Each lot of this antibody is quality control tested by ELISA. For ELISA detection, the suggested use of this reagent is ≤ 0.5 µg per ml. It's recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Kim HR, et al. 2010. Int Immunopharmacol. 10:1248. PubMedGrodeland G, et al. 2013. PLoS One. 11:80008. PubMed
Product Citations: Stoler-Barak L, et al. 2023. Nat Commun. 14:1462. PubMed Grødeland G, et al. 2013. PLoS One. 11:80008. PubMed Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed Lu X, et al. 2019. Cell Rep. 28:472. PubMed Kim H, et al. 2010. Int Immunopharmacol. 10:1242. PubMed
RRID: AB_315070 (BioLegend Cat. No. 406803)
Gene ID: 380795
UniProt: View information about IgG3 on UniProt.org
Clone: RMG3-1
Regulatory Status: RUO
Other Names: Immunoglobulin G3
Isotype: Rat IgG
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.