Biolegend, 407306, APC anti-mouse Ig light chain λ Antibody, 100μg

The RML-42 monoclonal antibody reacts with immunoglobulin light chain lambda in all tested mouse haplotype (Igh-a and b). It does not react with kappa chain. The RML-42 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Ota M, et al. 2010. J. Immunol. 185:4128. PubMed Xu Z, et al. 2012. J. Immunol. 189:4470. PubMed
Product Citations: Grenov A, et al. 2022. Cell Rep. 39:110778. PubMed Xu Z, et al. 2012. J Immunol. 189:4470. PubMed Peter AS, et al. 2021. Eur J Immunol. . PubMed Webster P, et al. 2018. Nat Commun. 9:2649. PubMed Blanc P, et al. 2016. Nat Commun. 7:13600. PubMed Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed Li X, et al. 2018. Immunity. 48:530. PubMed Ota M, et al. 2010. J Immunol. 185:4128. PubMed Tsyklauri O, et al. 2021. EMBO Rep. 22:e50785. PubMed Finkin S et al. 2019. Immunity. 51(2):324-336 . PubMed
RRID: AB_961363 (BioLegend Cat. No. 407306)
Biology Area: Immunology
Gene ID: 111519
UniProt: View information about Ig light chain lambda on UniProt.org
Clone: RML-42
Regulatory Status: RUO
Other Names: Imunoglobulin light chain lambda
Isotype: Rat IgG2a, κ