Biolegend, 407504, Biotin anti-rat IgG2a Antibody, 500μg

The MRG2a-83 monoclonal antibody reacts with rat immunoglobulin G2a (IgG2a) in all tested rat strains (Lou, Lou/Ws1/M, Lewis, Wistar, DA, Sprague-Dawley). It does not react with other isotypes. The MRG2a-83 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.
500μg
Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Mixed rat Igs
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: ELISA, FC
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Inoue S, et al. 2006. Cancer Research 66:7741.
Product Citations: Roth N, et al. 2022. Vaccines (Basel). 10:. PubMed
RRID: AB_345339 (BioLegend Cat. No. 407503) AB_345340 (BioLegend Cat. No. 407504)
Gene ID: 367586
UniProt: View information about IgG2a on UniProt.org
Clone: MRG2a-83
Regulatory Status: RUO
Other Names: Immunoglobulin G2a
Isotype: Mouse IgG
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.